PURE ZETA: PART FIVE: The PEG, ZETA POTENTIAL, AND THE FORMATION OF A NEW BIOLOGICAL IDENTITY:
How LNP can become a "PROTEIN CORONA", interact with the immune system, other parts of the body, cell receptors, and also lead to ADVERSE EVENTS AND INJURY (BATCH DEPENDENT!)
2/ The PEG is "steric barrier", like an umbrella (previous thread), to deter it from interacting w/ other molecules, such as charged particles (proteins can have charge!) It keeps it circulating longer too, as it is stealth from the immune system, like a cloaking device on a ship
3/ But, it can still interact with other things in the body. We know (TY SABINE!) that LNP is not forming evenly, and there are crystallization concerns (another thread), but also variations in PEG on outside of the LNP! This has many consequences for the LNP, and the human body.
4/ This study found extreme variances in amount of PEG on outside of the LNP, and ALSO If there was LESS PEG (PEG still has concerns) on outside of LNP that are shorter, these are correlated BY batch, as having HIGHER adverse events and injury to people.
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5/NEW BIOLGICAL IDENTITY AND SHIFTING ZETA POTENTIAL LEADING TO ADVERSE EVETS /INJURY
(COMBINED WITH DNA PLASMID CONTAMINATION):
The LNP has many parts as stated, but the photos that are displayed here are not representative of ALL LNPs, not by a long shot. They WILL VARY!
6/ (please excuse my poor drawings):
The Red backwards S is the modRNA (-) and the green (+) are positively charged, ionizable cationic lipids bound to each other [remember too, impurities in (+) lipids cause mutations (Packer et al., 2021)].
Purple lines will now be linear DNA
7/ However, "things" can stick to the outside of LNP, by attraction (charges--opposites attract), via van der Waals forces, absorption kinetics (higher amounts of proteins around LNP will attach to LNP FASTER), elevated temps, hydrophilic/hydrophobic interactions, causing a new
8/ entity to form. The study stated, the PEG was not present in the amounts it should be on the LNP. And with the DNA inside, the surface charge is already going to go MORE negative (possibly).This means it could stuck to proteins in the blood, such as albumin,.
(Albumin in red)
9/ Albumin is a protein in the blood. It has a negative charge. There are many things in the blood that can bind to an LNP (and in the body). When proteins stick to an LNP, this is called Opsonization. If albumin sticks to the LNP, it can then have an even HIGHER negative charge
10/ and cause MORE adverse events, such as clotting, immune reactions, Thrombosis and Hypercoagulability, Endothelial Dysfunction (clot formation again), Anti-phospholipid syndrome (APS), and binding with certain cell receptors!
Albumin specifically, makes up 60% of plasma.
11/ Albumin maintains osmotic pressure (oncotic pressure) in the bloodstream. This pressure gradient is essential for maintaining the balance of fluids between blood vessels and tissues, pH, and sodium levels! It acts as a buffer for pH in the blood, and also, can act as a ligand
12/ When the LNP goes even MORE negative, it can leak into the entire vascular when injected into the muscle (aspiration DOES NOT MATTER) and BIND to platelet factor 4 (PF4), causing CLOTS.
Also, Dr. G. Pain has been warning us about the endotoxin contamination because of the
13/ Use of plasmids not being filtered, which are "grown up" in E. coli, which is KNOWN to have bacteria called endotoxin. This bacteria ALSO HAS A NEGATIVE CHARGE and can stick to the outside of the LNP, also causing the ZETA POTENTIAL TO GO MORE NEGATIVE THAN BEFORE, AND take
14/ on an even wonkier biological identity, now able to cause even more clots, infection, high level immune response, change the properties of other things around it, cause adverse events, immune responses, and engage in different molecular responses in the body, causing harm.
15/ and because there is now a shift in zeta potential, the surface charge, it is going to move to different areas in the body---charge drives bio distribution AND interactions with cells in the body, and NOW it has proteins stuck to it, the peg, and bacteria--bad news bears.
16/ Without the PEG in full force on the outside of the LNP, it can also bind to ENDOTHELIAL CELLS. This can cause inflammation, immune response, vasoconstriction or vasodilation (causing vessels to constrict or contract), and express the spike protein in the endothelial, AND
17/ with PLASMID CONTAMINATION in the LNP, if THAT interacts with the endothelial cells, and transfects after binding because it became a protein corona, it could cause clots, mutation, disrupt endothelial unction, INFLAMMATION OF THE ENDOTHELIAL TISSUE,
18/ impacting the blood vessels, and the heart. This should be the primary mechanism for endothelial dysfunction, through initial contact with the endothelium, also would disrupt electrolyte balance in the body, negatively impact angiogenesis, and response to inflammation.
19/ As the LNP binds with multiple things, they can now form higher ordered structures, which are called complexes. Imagine, the LNP has a negative charge now, but then positive things can stick to that, and negative to that, like a chain, and start clumping.
The LNP can also
20/ form a protein corona by binding with immunoglobulins, Red Blood Cells (Erythrocytes) (leading to capillary blockages), white blood cells, Platelets (Thrombocytes)--leading to MORE clots (clot city here), and could cause additional bind events.
21/ Recently, over the weekend, McKernan, Speicher, and Rose spoke of their recent findings that DNA PLASMID contamination was correlated in VAERS to higher adverse events, finding higher amounts of DNA plasmid contamination was linked to VAERS records of injury from vaccination.
22/ Once again, this shoves the bolus theory from that guy overseas in the bin.
Because the PEG is not consistent in size or amount on the outside of the LNP, this is causing the surface charge of the LNP to shift, and to go either in the positive (+) or negative (+) which
23/ is changing the biodistribution, causing interactions and adverse events in multiple systems in the body, binding with proteins in the body, interacting with the immune system, causing clots, interacting with albumin, the endothelium and expressing the spike there, damaging
24/ it, while endotoxin drives it further negative if present, and DNA contamination being higher in VAERS batches ALL proves zeta potential is a primary driver of the acute adverse events (beyond spike, DNA potential for mutation, impurity in lipid driving mutation, and others)